UNIT 20.2 Analysis of Epigenetic Modifications of DNA in Human Cells
Published Online: 1 APR 2013
Copyright © 2013 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Kristensen, L. S., Treppendahl, M. B. and Grønbæk, K. 2013. Analysis of Epigenetic Modifications of DNA in Human Cells. Current Protocols in Human Genetics. 77:20.2:20.2.1–20.2.22.
- Published Online: 1 APR 2013
Epigenetics, the study of somatically heritable changes in gene expression not related to changes in the DNA sequence, is a rapidly expanding research field that plays important roles in healthy as well as in diseased cells. DNA methylation and hydroxymethylation are epigenetic modifications found in human cells, which are deeply implicated in normal cellular processes as well as in several major human diseases. Here, a range of different methods for the analyses of DNA methylation and hydroxymethylation at locus-specific and genome-wide scales is described. Curr. Protoc. Hum. Genet. 77:20.2.1-20.2.22. © 2013 by John Wiley & Sons, Inc.
- DNA methylation;
- DNA hydroxymethylation;
- methylation-specific PCR;
- methylation-independent PCR;
- genome-wide methylation analysis