APPENDIX 3E Enzymatic Labeling of DNA
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Tabor, S. and Struhl, K. 2001. Enzymatic Labeling of DNA. Current Protocols in Human Genetics. 00:3E:A.3E.1–A.3E.6.
- Published Online: 1 MAY 2001
- Published Print: FEB 1994
This appendix describes the preparation of uniformly labeled radioactive probes by nick translation and random oligonucleotide-primed synthesis, as well as the end-labeling of oligonucleotides by T4 polynucleotide kinase. Accompanying these protocols are methods for removing unincorporated dNTP precursors with spin columns and for measuring the specific activity of a probe by acid precipitation.