APPENDIX 3E Enzymatic Labeling of DNA

  1. Stanley Tabor,
  2. Kevin Struhl

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hga03es00

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Tabor, S. and Struhl, K. 2001. Enzymatic Labeling of DNA. Current Protocols in Human Genetics. 00:3E:A.3E.1–A.3E.6.

Author Information

  1. Harvard Medical School, Boston, Massachusetts

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: FEB 1994


This appendix describes the preparation of uniformly labeled radioactive probes by nick translation and random oligonucleotide-primed synthesis, as well as the end-labeling of oligonucleotides by T4 polynucleotide kinase. Accompanying these protocols are methods for removing unincorporated dNTP precursors with spin columns and for measuring the specific activity of a probe by acid precipitation.