Appendix

APPENDIX 3H Establishment of Permanent Cell Lines by Epstein-Barr Virus Transformation

  1. John Gilbert

Published Online: 1 MAY 2001

DOI: 10.1002/0471142905.hga03hs02

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Gilbert, J. 2001. Establishment of Permanent Cell Lines by Epstein-Barr Virus Transformation. Current Protocols in Human Genetics. 2:3H:A.3H.1–A.3H.5.

Author Information

  1. Duke University Medical Center, Durham, North Carolina

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: AUG 1994

Abstract

This basic protocol presents Epstein-Barr virus (EBV) transformation of B cells to create a permanent cell line to provide cells for genetic and DNA analysis. Virus stocks are prepared from cultures of an infected marmoset cell line in the first support protocol. Transformed cells may be analyzed immediately or frozen for future use as described in the second support protocol.