APPENDIX 3H Establishment of Permanent Cell Lines by Epstein-Barr Virus Transformation
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Gilbert, J. 2001. Establishment of Permanent Cell Lines by Epstein-Barr Virus Transformation. Current Protocols in Human Genetics. 2:3H:A.3H.1–A.3H.5.
- Published Online: 1 MAY 2001
- Published Print: AUG 1994
This basic protocol presents Epstein-Barr virus (EBV) transformation of B cells to create a permanent cell line to provide cells for genetic and DNA analysis. Virus stocks are prepared from cultures of an infected marmoset cell line in the first support protocol. Transformed cells may be analyzed immediately or frozen for future use as described in the second support protocol.