Appendix

APPENDIX 3L Introduction to Basic Mouse Handling Techniques

  1. John Donovan1,
  2. Patricia Brown2,
  3. J.P. Reeves3,
  4. P.A. Reeves3

Published Online: 1 MAY 2003

DOI: 10.1002/0471142905.hga03ls36

Current Protocols in Human Genetics

Current Protocols in Human Genetics

How to Cite

Donovan, J., Brown, P., Reeves, J. and Reeves, P. 2003. Introduction to Basic Mouse Handling Techniques. Current Protocols in Human Genetics. 36:3L:A.3L.1–A.3L.18.

Author Information

  1. 1

    Rhone-Poulenc Rorer, Collegeville, Pennsylvania

  2. 2

    National Cancer Institute, Bethesda, Maryland

  3. 3

    Food and Drug Administration, Bethesda, Maryland

Publication History

  1. Published Online: 1 MAY 2003
  2. Published Print: JAN 2003

Abstract

Purification of DNA from fixed, paraffin-embedded tissue (PET) requires the removal of paraffin, proteins, and, in some cases, heavy metal ions. The resulting DNA is not generally suitable for Southern blot analysis. However, the polymerase chain reaction (PCR) can be used to amplify short regions of this DNA. In this appendix, the basic protocol can be performed rapidly and involves only a small number of manipulations, thus minimizing the possibility of contamination. An alternate protocol involves additional steps including extractions with xylenes and phenol/chloroform. This procedure is recommended as the primary approach when tissue has not been fixed in an optimal manner or as a secondary approach when the basic protocol has failed to produce satisfactory DNA.