APPENDIX 3L Introduction to Basic Mouse Handling Techniques
Published Online: 1 MAY 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Human Genetics
How to Cite
Donovan, J., Brown, P., Reeves, J. and Reeves, P. 2003. Introduction to Basic Mouse Handling Techniques. Current Protocols in Human Genetics. 36:3L:A.3L.1–A.3L.18.
- Published Online: 1 MAY 2003
- Published Print: JAN 2003
Purification of DNA from fixed, paraffin-embedded tissue (PET) requires the removal of paraffin, proteins, and, in some cases, heavy metal ions. The resulting DNA is not generally suitable for Southern blot analysis. However, the polymerase chain reaction (PCR) can be used to amplify short regions of this DNA. In this appendix, the basic protocol can be performed rapidly and involves only a small number of manipulations, thus minimizing the possibility of contamination. An alternate protocol involves additional steps including extractions with xylenes and phenol/chloroform. This procedure is recommended as the primary approach when tissue has not been fixed in an optimal manner or as a secondary approach when the basic protocol has failed to produce satisfactory DNA.