UNIT 1.4 Establishing and Maintaining System Linearity

  1. James C. S. Wood

Published Online: 1 JAN 2009

DOI: 10.1002/0471142956.cy0104s47

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Wood, J. C. S. 2009. Establishing and Maintaining System Linearity. Current Protocols in Cytometry. 47:1.4:1.4.1–1.4.14.

Author Information

  1. Wake Forest University School of Medicine, Winston-Salem, North Carolina

Publication History

  1. Published Online: 1 JAN 2009
  2. Published Print: JAN 2009


A flow cytometer system transforms a received optical signal into an electrical signal whose amplitude ideally is directly proportional to the optical signal but in practice is at least related in a linear fashion. Establishment and maintenance of system linearity are prerequisites to accurate quantitative measurements. The many potential sources of offsets and nonlinearity are found in all parts of the detection, amplification, and data acquisition systems of a flow instrument. This unit aims to help investigators evaluate performance of the hardware in flow cytometer detection systems. Curr. Protocol. Cytom. 47:1.4.1-1.4.14. © 2009 by John Wiley & Sons, Inc.


  • flow cytometry;
  • linearity;
  • quantitation;
  • dynamic range;
  • proportional