UNIT 1.6 Laser Beam Shaping and Spot Size
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Shapiro, H. M. 2001. Laser Beam Shaping and Spot Size. Current Protocols in Cytometry. 1:1.6:1.6.1–1.6.5.
- Published Online: 1 MAY 2001
- Published Print: JUL 1997
One or more lasers are the source of illumination in the majority of fluorescence flow cytometers now in use. A typical laser emits a beam on the order of 1 mm in diameter. For efficient utilization this beam must be shaped and focused to a smaller size to illuminate the cell stream passing through the interrogation point. This unit discusses spot size requirements and the methods by which optimal spot size is achieved.