UNIT 1.7 High-Speed Cell Sorting
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Leary, J. F. 2001. High-Speed Cell Sorting. Current Protocols in Cytometry. 1:1.7:1.7.1–1.7.7.
- Published Online: 1 MAY 2001
- Published Print: JUL 1997
A flow cytometer has the ability to analyze several thousand cells per second. This is particularly valuable for the detection of rare events. For many biological and clinical applications, cells must be isolated on the basis of multiple quantitative properties. This need is ideally met by the analytical power of flow cytometry, which provides the front-end selection mechanism for cell sorting. In essence, cell sorting represents real-time data classification coupled with actual physical isolation of cells with the properties of interest. This unit discusses the principles and practices of operating a cell sorter to maximize the sorting rate, including the trade-offs between speed and resolution.