UNIT 1.9 Lasers for Flow Cytometry

  1. Howard M. Shapiro1,
  2. William G. Telford2

Published Online: 1 JUL 2009

DOI: 10.1002/0471142956.cy0109s49

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Shapiro, H. M. and Telford, W. G. 2009. Lasers for Flow Cytometry. Current Protocols in Cytometry. 49:1.9:1.9.1–1.9.17.

Author Information

  1. 1

    The Center for Microbial Cytometry, West Newton, Massachusetts

  2. 2

    Experimental Transplantation and Immunology Branch, National Cancer Institute, Bethesda, Maryland

Publication History

  1. Published Online: 1 JUL 2009
  2. Published Print: JUL 2009


Lasers are the principal light sources for flow cytometers. Most modern benchtop instruments feature air-cooled lasers emitting 10 to 25 mW of light at 488 nm. This unit covers the various types of lasers available and the qualities that make them suitable or unsuitable for use in flow cytometers. Also included is a discussion of future directions, particularly in the development of solid-state devices. Curr. Protoc. Cytom. 49:1.9.1-1.9.17. © 2009 by John Wiley & Sons, Inc.


  • flow cytometry;
  • Gaussian beams;
  • illumination optics;
  • lasers