UNIT 1.10 Techniques for Flow Cytometer Alignment

  1. James H. Jett,
  2. John C. Martin,
  3. Robert C. Habbersett

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0110s06

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Jett, J. H., Martin, J. C. and Habbersett, R. C. 2001. Techniques for Flow Cytometer Alignment. Current Protocols in Cytometry. 6:1.10:1.10.1–1.10.6.

Author Information

  1. Los Alamos National Laboratory, Los Alamos, New Mexico

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1998

This is not the most recent version of the article. View current version (1 OCT 2009)


This unit provides essential knowledge for correctly using any flow cytometer to ensure that data collected are accurate and reliable. Two levels of system alignment are described: routine alignment checks and complete alignment, both of which can be applied to a variety of instrument configurations.