UNIT 1.10 Techniques for Flow Cytometer Alignment

  1. James H. Jett,
  2. John C. Martin,
  3. Robert C. Habbersett

Published Online: 1 OCT 2009

DOI: 10.1002/0471142956.cy0110s50

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Jett, J. H., Martin, J. C. and Habbersett, R. C. 2009. Techniques for Flow Cytometer Alignment. Current Protocols in Cytometry. 50:1.10:1.10.1–1.10.7.

Author Information

  1. National Flow Cytometry Resource, Bioscience Division, Los Alamos National Laboratory, Los Alamos, New Mexico

Publication History

  1. Published Online: 1 OCT 2009
  2. Published Print: OCT 2009


This unit provides essential knowledge for correctly using any flow cytometer to ensure that data collected are accurate and reliable. Two levels of system alignment are described: routine alignment checks and complete alignment, both of which can be applied to a variety of instrument configurations. Curr. Protoc. Cytom. 50:1.10.1-1.10.7. © 2009 by John Wiley & Sons, Inc.


  • flow cytometer alignment;
  • laser beam profile analysis;
  • oscilloscope