Unit

UNIT 1.12 Principles of Resonance Energy Transfer

  1. János Szöllősi,
  2. Sándor Damjanovich,
  3. László Mátyus

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0112s09

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Szöllősi, J., Damjanovich, S. and Mátyus, L. 2001. Principles of Resonance Energy Transfer. Current Protocols in Cytometry. 1:1.12.

Author Information

  1. University Medical School of Debrecen, Debrecen, Hungary

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1999

This is not the most recent version of the article. View current version (1 NOV 2006)

Abstract

Fluorescence Resonance Energy Transfer (FRET) is a radiationless process in which energy is transferred from an excited donor to an acceptor molecule. For many years this principle has been exploited in flow cytometry to excite long wavelength fluorophores with short wavelength excitation; the donor/acceptor molecules are frequently known as tandem conjugates. This unit explains in careful detail the quite complex FRET process. It characterizes available parameters and instruments for FRET measurements, discusses limitations, and shows a few examples for the application of FRET, such as determining occupation of closely related receptors.