UNIT 1.12 Principles of Resonance Energy Transfer
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Szöllősi, J., Damjanovich, S. and Mátyus, L. 2001. Principles of Resonance Energy Transfer. Current Protocols in Cytometry. 1:1.12.
- Published Online: 1 MAY 2001
- Published Print: JUL 1999
This is not the most recent version of the article. View current version (1 NOV 2006)
Fluorescence Resonance Energy Transfer (FRET) is a radiationless process in which energy is transferred from an excited donor to an acceptor molecule. For many years this principle has been exploited in flow cytometry to excite long wavelength fluorophores with short wavelength excitation; the donor/acceptor molecules are frequently known as tandem conjugates. This unit explains in careful detail the quite complex FRET process. It characterizes available parameters and instruments for FRET measurements, discusses limitations, and shows a few examples for the application of FRET, such as determining occupation of closely related receptors.