Unit

UNIT 1.17 Plug Flow Cytometry

  1. Bruce S. Edwards1,
  2. Larry A. Sklar1,2

Published Online: 1 AUG 2001

DOI: 10.1002/0471142956.cy0117s17

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Edwards, B. S. and Sklar, L. A. 2001. Plug Flow Cytometry. Current Protocols in Cytometry. 17:1.17:1.17.1–1.17.10.

Author Information

  1. 1

    University of New Mexico, Albuquerque, New Mexico

  2. 2

    Los Alamos National Laboratory, National Flow Cytometry Resource, Los Alamos, New Mexico

Publication History

  1. Published Online: 1 AUG 2001
  2. Published Print: JUL 2001

Abstract

Although flow cytometry has the powerful ability to rapidly screen large collections of cells, the technology has yet to be efficiently applied to large-scale screening operations involving multiple discrete suspensions. High-throughput flow cytometry would be beneficial to many areas of biological investigation, such as modern drug discovery, which involves testing of cellular targets against millions of potentially valuable compounds. The authors have developed a flow injection analysis approach to automated sample handling in which individual sample suspensions are sequentially inserted as plugs of precisely defined volumes into a flowing fluid which delivers them to the laser beam. The unit describes the basic elements and concepts of this plug flow system and discusses representative applications.