UNIT 1.18 Dynamic Thermoregulation of the Sample in Flow Cytometry

  1. Steven W. Graves,
  2. Robert C. Habbersett,
  3. John P. Nolan

Published Online: 1 MAY 2002

DOI: 10.1002/0471142956.cy0118s20

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Graves, S. W., Habbersett, R. C. and Nolan, J. P. 2002. Dynamic Thermoregulation of the Sample in Flow Cytometry. Current Protocols in Cytometry. 20:1.18:1.18.1–1.18.16.

Author Information

  1. Los Alamos National Laboratories, Los Alamos, New Mexico

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: JUL 2002


Fine control of temperature is an important capability for any analytical platform. A circulating water bath has been the traditional means of maintaining constant temperature in the sample chamber of a flow cytometer, but this approach does not permit rapid changes in sample temperature. This unit explains the use of Peltier modules for regulation of sample temperature. The heat pumping generated by the passage of current through properly matched semiconductors, known as the Peltier effect, makes it possible for these thermoelectric modules to both heat and cool. The authors describe the construction of a Peltier module based thermoregulation unit in step-by-step detail and present a demonstration of flow cytometry measurements as a function of temperature.