UNIT 1.18 Dynamic Thermoregulation of the Sample in Flow Cytometry
Published Online: 1 MAY 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Graves, S. W., Habbersett, R. C. and Nolan, J. P. 2002. Dynamic Thermoregulation of the Sample in Flow Cytometry. Current Protocols in Cytometry. 20:1.18:1.18.1–1.18.16.
- Published Online: 1 MAY 2002
- Published Print: JUL 2002
Fine control of temperature is an important capability for any analytical platform. A circulating water bath has been the traditional means of maintaining constant temperature in the sample chamber of a flow cytometer, but this approach does not permit rapid changes in sample temperature. This unit explains the use of Peltier modules for regulation of sample temperature. The heat pumping generated by the passage of current through properly matched semiconductors, known as the Peltier effect, makes it possible for these thermoelectric modules to both heat and cool. The authors describe the construction of a Peltier module based thermoregulation unit in step-by-step detail and present a demonstration of flow cytometry measurements as a function of temperature.