Unit

UNIT 1.21 Separation Index: An Easy-to-Use Metric for Evaluation of Different Configurations on the Same Flow Cytometer

  1. Martin Bigos

Published Online: 1 APR 2007

DOI: 10.1002/0471142956.cy0121s40

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Bigos, M. 2007. Separation Index: An Easy-to-Use Metric for Evaluation of Different Configurations on the Same Flow Cytometer. Current Protocols in Cytometry. 40:1.21:1.21.1–1.21.6.

Author Information

  1. Gladstone Institute of Virology and Immunology, San Francisco, California

Publication History

  1. Published Online: 1 APR 2007
  2. Published Print: APR 2007

Abstract

A quick and understandable method, using material readily available in a biology laboratory, for evaluating the measurement sensitivity of flow cytometers is presented in detail. Two examples of its use, in determining an optimal emission filter and in evaluating compensation, are also presented.

Keywords:

  • configuration;
  • filters;
  • sensitivity;
  • evaluation