UNIT 1.21 Separation Index: An Easy-to-Use Metric for Evaluation of Different Configurations on the Same Flow Cytometer
Published Online: 1 APR 2007
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Bigos, M. 2007. Separation Index: An Easy-to-Use Metric for Evaluation of Different Configurations on the Same Flow Cytometer. Current Protocols in Cytometry. 40:1.21:1.21.1–1.21.6.
- Published Online: 1 APR 2007
- Published Print: APR 2007
A quick and understandable method, using material readily available in a biology laboratory, for evaluating the measurement sensitivity of flow cytometers is presented in detail. Two examples of its use, in determining an optimal emission filter and in evaluating compensation, are also presented.