UNIT 1.22 Fundamentals of Acoustic Cytometry

  1. Michael Ward1,
  2. Patrick Turner2,
  3. Marc DeJohn3,
  4. Gregory Kaduchak1

Published Online: 1 JUL 2009

DOI: 10.1002/0471142956.cy0122s49

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Ward, M., Turner, P., DeJohn, M. and Kaduchak, G. 2009. Fundamentals of Acoustic Cytometry. Current Protocols in Cytometry. 53:1.22:1.22.1–1.22.12.

Author Information

  1. 1

    Life Technologies, Eugene, Oregon

  2. 2

    Los Alamos Technical Services, Los Alamos, New Mexico

  3. 3

    Santa Fe Technical Services, Santa Fe, New Mexico

Publication History

  1. Published Online: 1 JUL 2009
  2. Published Print: JUL 2009


Acoustic cytometry is a new technology that replaces or partly replaces hydrodynamic focusing of cells or particles with focusing derived from acoustic radiation pressure forces. It offers new possibilities for improving current flow cytometry assays and creating new ones. To take full advantage of these possibilities, it is necessary to understand the fundamental benefits and limitations of acoustic focusing as employed in flow cytometry analysis, either as a substitute for hydrodynamic focusing or in combination with it. Curr. Protoc. Cytom. 49:1.22.1-1.22.12. © 2009 by John Wiley & Sons, Inc.


  • flow cytometry;
  • acoustic focusing;
  • sample preparation