UNIT 2.1 Contrast Enhancement in Light Microscopy
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Keller, H. E. 2001. Contrast Enhancement in Light Microscopy. Current Protocols in Cytometry. 00:2.1:2.1.1–2.1.11.
- Published Online: 1 MAY 2001
- Published Print: APR 1997
This is not the most recent version of the article. View current version (1 JAN 2013)
The optical microscope is a fundamental component of an image cytometry system. Electronic imaging has both greatly enhanced the capabilities of light microscopes and placed ever-increasing demands on their optical performance. This unit covers the basic concepts of light microscopy, including Köhler illumination, resolution, contrast, and numerical aperture, and reviews the many types of instruments and techniques for contrast enhancement.