UNIT 2.2 Microscope Objectives

  1. Mortimer Abramowitz1,
  2. Marc M. Friedman2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0202s00

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Abramowitz, M. and Friedman, M. M. 2001. Microscope Objectives. Current Protocols in Cytometry. 00:2.2:2.2.1–2.2.14.

Author Information

  1. 1

    Olympus America Inc., Melville, New York

  2. 2

    AccuMed International, Chicago, Illinois

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1997

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The objective is the most crucial image-forming component of a microscope. A knowledge of the many types of objectives available and their characteristics is critical to the selection of appropriate objectives for image cytometry. This unit discusses aberrations in image formation and their correction, construction and types of objectives, and objectives for other microscopy applications, explaining the advantages and limitations of each one.