UNIT 2.2 Microscope Objectives

  1. Joseph LoBiondo1,
  2. Mortimer Abramowitz2,
  3. Marc M. Friedman3

Published Online: 1 OCT 2011

DOI: 10.1002/0471142956.cy0202s58

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

LoBiondo, J., Abramowitz, M. and Friedman, M. M. 2011. Microscope Objectives. Current Protocols in Cytometry. 58:2.2:2.2.1–2.2.15.

Author Information

  1. 1

    Nikon Instruments, Melville, New York

  2. 2

    Olympus America, Central Valley, Pennsylvania

  3. 3

    AccuMed International, Chicago, Illinois

Publication History

  1. Published Online: 1 OCT 2011
  2. Published Print: OCT 2011


The objective is the most crucial image-forming component of a microscope. A knowledge of the many types of objectives available and their characteristics is critical to the selection of appropriate objectives for image cytometry. This unit discusses aberrations in image formation and their correction, construction, and types of objectives, and objectives for other microscopy applications, explaining the advantages and limitations of each one. Curr. Protoc. Cytom. 58:2.2.1-2.2.15. © 2011 by John Wiley & Sons, Inc.


  • imaging and microscopy;
  • confocal microscopy;
  • light microscopy