UNIT 2.3 Light Microscopy Digital Imaging

  1. John Ossi

Published Online: 1 OCT 2008

DOI: 10.1002/0471142956.cy0203s46

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Ossi, J. 2008. Light Microscopy Digital Imaging. Current Protocols in Cytometry. 46:2.3:2.3.1–2.3.10.

Author Information

  1. Quantitative Imaging, Germantown, Maryland

Publication History

  1. Published Online: 1 OCT 2008
  2. Published Print: OCT 2008

This is not the most recent version of the article. View current version (1 OCT 2011)


This unit presents an overview of digital imaging hardware used in light microscopy. CMOS, CCD, and EMCCDs are the primary sensors used. The strengths and weaknesses of each define the primary applications for these sensors. Sensor architecture and formats are also reviewed. Color camera design strategies and sensor window cleaning are also described in the unit. Curr. Protocol. Cytom. 46:2.3.1-2.3.10. © 2008 by John Wiley & Sons, Inc.


  • microscopy;
  • digital;
  • imaging