UNIT 2.3 Light Microscopy Digital Imaging

  1. James Joubert,
  2. Deepak Sharma

Published Online: 1 OCT 2011

DOI: 10.1002/0471142956.cy0203s58

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Joubert, J. and Sharma, D. 2011. Light Microscopy Digital Imaging. Current Protocols in Cytometry. 58:2.3:2.3.1–2.3.11.

Author Information

  1. Photometrics, Tucson, Arizona

Publication History

  1. Published Online: 1 OCT 2011
  2. Published Print: OCT 2011


This unit presents an overview of digital imaging hardware used in light microscopy. CMOS, CCD, and EMCCDs are the primary sensors used. The strengths and weaknesses of each define the primary applications for these sensors. Sensor architecture and formats are also reviewed. Color camera design strategies and sensor window cleaning are also described in the unit. Curr. Protoc. Cytom. 58:2.3.1-2.3.11. © 2011 by John Wiley & Sons, Inc.


  • microscopy;
  • digital;
  • imaging