UNIT 2.4 Optical Filtering Systems for Wavelength Selection in Light Microscopy

  1. Alan S. Waggoner1,
  2. Elliot S. Wachman1,
  3. Daniel L. Farkas2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0204s15

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Waggoner, A. S., Wachman, E. S. and Farkas, D. L. 2001. Optical Filtering Systems for Wavelength Selection in Light Microscopy. Current Protocols in Cytometry. 15:2.4:2.4.1–2.4.11.

Author Information

  1. 1

    Carnegie Mellon University, Pittsburgh, Pennsylvania

  2. 2

    Carnegie Mellon University and University of Pittsburgh, Pittsburgh, Pennsylvania

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2001

This is not the most recent version of the article. View current version (1 APR 2011)


Because of its sensitivity and specificity, fluorescence-based detection is one of the foremost methods for microscopic imaging of biological tissues and cells. Dramatic improvements in filter system design and implementation coupled with development of an ever-widening range of sensitive fluorescent dyes have made multicolor imaging a powerful tool for structural and functional analysis. This revised and expanded unit reviews some of the main principles and developments of optical filtering.