UNIT 2.5 Digital Fluorescence Microscopy

  1. Hans J. Tanke

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0205s00

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Tanke, H. J. 2001. Digital Fluorescence Microscopy. Current Protocols in Cytometry. 00:2.5:2.5.1–2.5.5.

Author Information

  1. Leiden University, Leiden, The Netherlands

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1997


Fluorescence microscopy is a valuable tool for biomedical research, providing good sensitivity and high multiplicity. Specialized techniques (anisotropy measurement, resonance energy transfer) can provide information on cell spatial arrangement or label microenvironment. The unit discusses camera selection strategy, microscope hardware, and image acquisition and processing.