UNIT 2.7 Microscope Alignment

  1. Marc M. Friedman1,
  2. Mortimer Abramowitz2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0207s02

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Friedman, M. M. and Abramowitz, M. 2001. Microscope Alignment. Current Protocols in Cytometry. 2:2.7:2.7.1–2.7.8.

Author Information

  1. 1

    Accumed International, Inc., Chicago, Illinois

  2. 2

    Olympus America Inc., Mellville, New York

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1997


In order to obtain good images using any microscopic imaging modalities, it is necessary to understand the microscope thoroughly and set up properly. This unit should be required reading for all students and staff using a light microscope. It clearly defines the types of illumination used in microscopes and details the correct set up and operation.

Keywords: light microscopes; Köhler illumination; microscope alignment.