UNIT 2.11 Shading Correction: Compensation for Illumination and Sensor Inhomogeneities

  1. Ian T. Young

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0211s14

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Young, I. T. 2001. Shading Correction: Compensation for Illumination and Sensor Inhomogeneities. Current Protocols in Cytometry. 14:2.11:2.11.1–2.11.12.

Author Information

  1. Delft University of Technology, Delft, The Netherlands

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 2000


The interaction between objects in real space, the illumination, and the camera frequently leads to a situation in which a microscope image exhibits significant shading across the field of view. In general this shading effect is undesirable and requires elimination, especially for quantitative microscopy. Starting with a simple mathematical model, this unit develops procedures to correct images for the shading thus introduced in the image-formation process. Two cases are distinguished: the a priori, which assumes the availability of calibration images in addition to the images of interest; and the a posteriori, which assumes that only the recorded images of interest are available.