UNIT 2.14 Methods and Applications of Laser-Enabled Analysis and Processing (LEAP)

  1. Nan Lin,
  2. Jennifer R. Cresswell,
  3. Genova A. Richardson,
  4. Mark A. Gerber,
  5. Kevin J. Kayser

Published Online: 1 JAN 2008

DOI: 10.1002/0471142956.cy0214s43

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Lin, N., Cresswell, J. R., Richardson, G. A., Gerber, M. A. and Kayser, K. J. 2008. Methods and Applications of Laser-Enabled Analysis and Processing (LEAP). Current Protocols in Cytometry. 43:2.14:2.14.1–2.14.27.

Author Information

  1. Cell Sciences and Development, SAFC Biosciences, St. Louis, Missouri

Publication History

  1. Published Online: 1 JAN 2008
  2. Published Print: JAN 2008


The LEAP (Laser-Enabled Analysis and Processing) platform combines in situ imaging with laser manipulation to efficiently identify, purify, and monitor expansion of high secreting clones. It also allows for rapid analysis of cell population heterogeneity. This unit describes the LEAP instrumentation as well as basic and alternate protocols of the major applications in recombinant human or humanized IgG expression. The protocols include fluorescent cell counting, secreted recombinant IgG capture and detection, and IgG-secreting clone selection by laser processing. Curr. Protocol. Cytom. 43:2.14.1-2.14.27. © 2008 by John Wiley & Sons, Inc.


  • Laser-Enabled Analysis and Processing (LEAP);
  • recombinant IgG production;
  • high-producing cell line generation;
  • in situ detection;
  • secretion heterogeneity