UNIT 2.14 Methods and Applications of Laser-Enabled Analysis and Processing (LEAP)
Published Online: 1 JAN 2008
Copyright © 2007 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Lin, N., Cresswell, J. R., Richardson, G. A., Gerber, M. A. and Kayser, K. J. 2008. Methods and Applications of Laser-Enabled Analysis and Processing (LEAP). Current Protocols in Cytometry. 43:2.14:2.14.1–2.14.27.
- Published Online: 1 JAN 2008
- Published Print: JAN 2008
The LEAP (Laser-Enabled Analysis and Processing) platform combines in situ imaging with laser manipulation to efficiently identify, purify, and monitor expansion of high secreting clones. It also allows for rapid analysis of cell population heterogeneity. This unit describes the LEAP instrumentation as well as basic and alternate protocols of the major applications in recombinant human or humanized IgG expression. The protocols include fluorescent cell counting, secreted recombinant IgG capture and detection, and IgG-secreting clone selection by laser processing. Curr. Protocol. Cytom. 43:2.14.1-2.14.27. © 2008 by John Wiley & Sons, Inc.
- Laser-Enabled Analysis and Processing (LEAP);
- recombinant IgG production;
- high-producing cell line generation;
- in situ detection;
- secretion heterogeneity