UNIT 2.22 How to Build a Time-Gated Luminescence Microscope
Published Online: 2 JAN 2014
Copyright © 2001 John Wiley & Sons, Inc. All rights reserved.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Jin, D., Lu, Y., Leif, R. C., Yang, S., Rajendran, M. and Miller, L. W. 2014. How to Build a Time-Gated Luminescence Microscope. Current Protocols in Cytometry. 67:2.22:2.22.1–2.22.36.
- Published Online: 2 JAN 2014
The sensitivity of filter-based fluorescence microscopy techniques is limited by autofluorescence background. Time-gated detection is a practical way to suppress autofluorescence, enabling higher contrast and improved sensitivity. In the past few years, three groups of authors have demonstrated independent approaches to build robust versions of time-gated luminescence microscopes. Three detailed, step-by-step protocols are provided here for modifying standard fluorescent microscopes to permit imaging time-gated luminescence. Curr. Protoc. Cytom. 67:2.22.1-2.22.36. © 2014 by John Wiley & Sons, Inc.
- time-gated luminescence;