UNIT 3.4 Safe Use of Hazardous Chemicals

  1. George Lunn1,
  2. Gretchen Lawler (chemical resistance of gloves)2

Published Online: 1 MAY 2006

DOI: 10.1002/0471142956.cy0304s36

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Lunn, G. and Lawler, G. 2006. Safe Use of Hazardous Chemicals. Current Protocols in Cytometry. 36:3.4:3.4.1–3.4.38.

Author Information

  1. 1

    Baltimore, Maryland

  2. 2

    Purdue University, West Lafayette, Indiana

Publication History

  1. Published Online: 1 MAY 2006
  2. Published Print: APR 2006


This appendix provides protocols for some commonly used disposal and decontamination procedures along with analytical techniques that are used to verify that reagents have been decontaminated. Some of the specific reagents covered are diaminobenzidine, ethidium bromide, cyanogen bromide and chloromethylsilane. With modification, these assays may also be used to determine the concentration of a particular chemical. Precautions are also detailed for routine handling of viable pathogenic microorganisms, as well as all human-derived materials, because they may harbor dangerous pathogens such as human immunodeficiency virus (HIV), hepatitis B virus (HBV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), and a host of bacterial pathogens.