Unit

UNIT 3.5 Method for Visualizing Aerosol Contamination in Flow Sorters

  1. Andrew S. Oberyszyn

Published Online: 1 FEB 2002

DOI: 10.1002/0471142956.cy0305s19

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Oberyszyn, A. S. 2002. Method for Visualizing Aerosol Contamination in Flow Sorters. Current Protocols in Cytometry. 19:3.5:3.5.1–3.5.7.

Author Information

  1. The Ohio State University Comprehensive Cancer Center, Analytical Cytometry Shared Resource Laboratory, Ohio State University, Columbus, Ohio

Publication History

  1. Published Online: 1 FEB 2002
  2. Published Print: JAN 2002

Abstract

The routine operation of a flow cytometer generates aerosols, and most commercially available instrument are not designed to be 100% effective in their elimination. Aerosols are a concern for operators as well as for others in the facility because of the potential for exposure to contamination, especially during sorting of biohazardous samples. This unit describes a visual method for examining aerosol containment using a commercial resin that fluoresces under UV or black light illumination. The method is rapid, inexpensive, and simple. Results are immediate and potentially quantitative