UNIT 3.5 Method for Visualizing Aerosol Contamination in Flow Sorters
Published Online: 1 FEB 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Oberyszyn, A. S. 2002. Method for Visualizing Aerosol Contamination in Flow Sorters. Current Protocols in Cytometry. 19:3.5:3.5.1–3.5.7.
- Published Online: 1 FEB 2002
- Published Print: JAN 2002
The routine operation of a flow cytometer generates aerosols, and most commercially available instrument are not designed to be 100% effective in their elimination. Aerosols are a concern for operators as well as for others in the facility because of the potential for exposure to contamination, especially during sorting of biohazardous samples. This unit describes a visual method for examining aerosol containment using a commercial resin that fluoresces under UV or black light illumination. The method is rapid, inexpensive, and simple. Results are immediate and potentially quantitative