UNIT 4.1 Titering Antibodies

  1. Carleton C. Stewart,
  2. Sigrid J. Stewart

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0401s14

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Stewart, C. C. and Stewart, S. J. 2001. Titering Antibodies. Current Protocols in Cytometry. 00:4.1:4.1.1–4.1.13.

Author Information

  1. Roswell Park Cancer Institute, Buffalo, New York

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 2000


Flow cytometry using fluorochrome-conjugated antibodies has emerged as a major approach to automated cellular identification. One of the most important issues in immunophenotyping is using the correct amount of antibody. This unit presents techniques for ascertaining the optimal titer for individual, dual, and multiple antibodies used for simultaneous phenotyping, stressing the importance of quality control in making batches of antibody for routine use. Keywords: flow cytometry; monoclonal antibodies; antibody titration; immunophenotyping.