Unit

UNIT 4.2 Conjugation of Fluorochromes to Monoclonal Antibodies

  1. Kevin L. Holmes1,
  2. Larry M. Lantz1,
  3. Wesley Russ2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0402s00

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Holmes, K. L., Lantz, L. M. and Russ, W. 2001. Conjugation of Fluorochromes to Monoclonal Antibodies. Current Protocols in Cytometry. 00:4.2:4.2.1–4.2.12.

Author Information

  1. 1

    National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

  2. 2

    Kirkegaard & Perry Laboratories, Gaithersburg, Maryland

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1997

Abstract

Detection of cell surface molecules labeled by monoclonal or polyclonal antibodies conjugated to a fluorochrome is probably the most widely used application of flow cytometry. This unit contains protocols for tagging monoclonal antibodies with fluorescein, biotin, Texas Red, and phycobiliproteins. In addition, it provides a procedure for preparing a PE-Texas Red tandem conjugate dye that can then be used for antibody conjugation. These protocols enable investigators to label antibodies of their choice with multiple fluorochromes and permit more combinations of antibodies for multicolor flow applications.

Keywords: flow cytometry; monoclonal antibodies; fluorochromes; antibody labeling.