UNIT 4.2 Conjugation of Fluorochromes to Monoclonal Antibodies
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Holmes, K. L., Lantz, L. M. and Russ, W. 2001. Conjugation of Fluorochromes to Monoclonal Antibodies. Current Protocols in Cytometry. 00:4.2:4.2.1–4.2.12.
- Published Online: 1 MAY 2001
- Published Print: APR 1997
Detection of cell surface molecules labeled by monoclonal or polyclonal antibodies conjugated to a fluorochrome is probably the most widely used application of flow cytometry. This unit contains protocols for tagging monoclonal antibodies with fluorescein, biotin, Texas Red, and phycobiliproteins. In addition, it provides a procedure for preparing a PE-Texas Red tandem conjugate dye that can then be used for antibody conjugation. These protocols enable investigators to label antibodies of their choice with multiple fluorochromes and permit more combinations of antibodies for multicolor flow applications.
Keywords: flow cytometry; monoclonal antibodies; fluorochromes; antibody labeling.