UNIT 4.3 Nucleic Acid Probes

  1. Martin Poot

Published Online: 1 NOV 2004

DOI: 10.1002/0471142956.cy0403s26

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Poot, M. 2004. Nucleic Acid Probes. Current Protocols in Cytometry. 26:4.3:4.3.1–4.3.10.

Author Information

  1. University of Washington Seattle, Seattle, Washington

Publication History

  1. Published Online: 1 NOV 2004
  2. Published Print: OCT 2003


This unit describes the physicochemical structures of commonly used nucleic acid probes, classified into four groups based on their mode of nucleic acid binding. The unit provides an excellent background for the many protocols employing these dyes and should be a prerequisite for any studies involving nuclear probes.


  • nucleic acid probes;
  • intercalating dyes;
  • minor groove-binding dyes;
  • metachromatic dyes;
  • DNA stains;
  • physicochemical structures