Unit

UNIT 4.6 Flow Cytometric Sorting of Bacterial Surface-Displayed Libraries

  1. Sophia Kenrick,
  2. Jeffrey Rice,
  3. Patrick Daugherty

Published Online: 1 OCT 2007

DOI: 10.1002/0471142956.cy0406s42

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Kenrick, S., Rice, J. and Daugherty, P. 2007. Flow Cytometric Sorting of Bacterial Surface-Displayed Libraries. Current Protocols in Cytometry. 42:4.6:4.6.1–4.6.27.

Author Information

  1. University of California, Santa Barbara, California

Publication History

  1. Published Online: 1 OCT 2007
  2. Published Print: OCT 2007

Abstract

The protocols herein detail methods for isolating binding peptides from a combinatorial library displayed on the surface of bacterial cells. These methods are appropriate for a variety of display scaffolds and a large range of library sizes, up to ∼5 × 109 or more. Instructions have been provided for isolating peptides that bind to both proteins and non-protein targets, such as whole cells or inorganic particles. Qualitative analysis by flow cytometry can be exploited for bacterial libraries to characterize a displayed peptide's binding properties with a target of interest, and sorting conditions can be tuned to maximize binding affinity. Curr. Protocol. Cytom. 42:4.6.1-4.6.27. © 2007 by John Wiley & Sons, Inc.

Keywords:

  • bacterial libraries;
  • surface display;
  • peptide;
  • binding;
  • ligand;
  • E. coli