UNIT 5.1 Handling, Storage, and Preparation of Human Blood Cells

  1. J. Philip McCoy, Jr.

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0501s00

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

McCoy, Jr., J. P. 2001. Handling, Storage, and Preparation of Human Blood Cells. Current Protocols in Cytometry. 5:5.1:5.1.1–5.1.13.

Author Information

  1. Cooper Hospital/University Medical Center, Camden, New Jersey

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1997

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Human peripheral blood samples are probably the most common specimens submitted for flow cytometric analysis. Handling and preparation will vary depending on the cell lineage to be examined and the type of assay. This unit presents protocols for separating white cells by lysing erythrocytes, isolating mononuclear cells by trilineage separation, and assorted methods for isolating or enriching specific cell populations, including monocytes, neutrophils, T lymphocytes, B lymphocytes, NK cells, stem cells, and platelets. Keywords: flow cytometry; human blood; leukocyte preparation; mononuclear blood cells; cell population enrichment; cell population isolation; cell population purification