Unit

UNIT 5.2 Handling, Storage, and Preparation of Human Tissues

  1. Lynn G. Dressler1,
  2. Dan Visscher2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0502s01

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Dressler, L. G. and Visscher, D. 2001. Handling, Storage, and Preparation of Human Tissues. Current Protocols in Cytometry. 1:5.2:5.2.1–5.2.15.

Author Information

  1. 1

    University of North Carolina, Chapel Hill, North Carolina

  2. 2

    Harper Hospital, Detroit, Michigan

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1997

Abstract

Human tissue for flow cytometry must be prepared as an adequate single-cell suspension. The appropriate methods for tissue collection, transport, storage, and dissociation depend on the cell parameters being measured and the localization of the markers. This unit includes a general method for collecting and transporting human tissue and preparing a tissue imprint. Protocols are supplied for tissue disaggregation by either mechanical or enzymatic means and for preparation of single-cell suspensions of whole cells from fine-needle aspirates, pleural effusions, abdominal fluids, or other body fluids. Other protocols detail preparation of intact nuclei from fresh, frozen, or paraffin-embedded tissue. Support protocols cover fixation, cryospin preparation, cryopreservation, and removal of debris. Keywords: flow cytometry; human tissue; tissue imprint; single-cell suspension preparation; tissue disaggregation; paraffin-embedded tissue