UNIT 5.2 Handling, Storage, and Preparation of Human Tissues
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Dressler, L. G. and Visscher, D. 2001. Handling, Storage, and Preparation of Human Tissues. Current Protocols in Cytometry. 1:5.2:5.2.1–5.2.15.
- Published Online: 1 MAY 2001
- Published Print: JUL 1997
Human tissue for flow cytometry must be prepared as an adequate single-cell suspension. The appropriate methods for tissue collection, transport, storage, and dissociation depend on the cell parameters being measured and the localization of the markers. This unit includes a general method for collecting and transporting human tissue and preparing a tissue imprint. Protocols are supplied for tissue disaggregation by either mechanical or enzymatic means and for preparation of single-cell suspensions of whole cells from fine-needle aspirates, pleural effusions, abdominal fluids, or other body fluids. Other protocols detail preparation of intact nuclei from fresh, frozen, or paraffin-embedded tissue. Support protocols cover fixation, cryospin preparation, cryopreservation, and removal of debris. Keywords: flow cytometry; human tissue; tissue imprint; single-cell suspension preparation; tissue disaggregation; paraffin-embedded tissue