Unit

UNIT 5.3 Flow Analysis and Sorting of Plant Chromosomes

  1. Jaroslav Doležel1,
  2. Jiří Macas2,
  3. Sergio Lucretti3

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0503s09

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Doležel, J., Macas, J. and Lucretti, S. 2001. Flow Analysis and Sorting of Plant Chromosomes. Current Protocols in Cytometry. 9:5.3:5.3.1–5.3.33.

Author Information

  1. 1

    Institute of Experimental Botany, Olomouc, Czech Republic

  2. 2

    Institute of Plant Molecular Biology, Ceské Budejovice, Czech Republic

  3. 3

    ENEA, Casaccia Research Center, Rome, Italy

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JUL 1999

Abstract

The use of flow cytometry for evaluation of plant chromosomes requires some specialized attention to preparation and instrumentation. This unit deals exclusively with plant cytogenetics and presents an outline of this area as well as methods for accumulation of cells in metaphase, preparation of chromosome suspensions, flow analysis and sorting of chromosomes, and processing of the sorted chromosomes. Each method is described in tremendous detail because in many aspects dealing with plant cells is quite different from dealing with mammalian cells. Supporting histograms are presented as well as a range of special hints on dealing with plant material and a discussion of the utility of sorted chromosomes for plant genome mapping.. Keywords: flow cytometry; plant chromosomes; cell cycle synchronization; chromosome isolation; chromosome sorting; physical gene mapping; chromosome-specific DNA libraries