Unit

UNIT 6.4 Enumeration of CD34+ Hematopoietic Stem and Progenitor Cells

  1. D. Robert Sutherland1,
  2. Michael Keeney2,
  3. Jan W. Gratama3

Published Online: 1 AUG 2003

DOI: 10.1002/0471142956.cy0604s25

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Sutherland, D. R., Keeney, M. and Gratama, J. W. 2003. Enumeration of CD34+ Hematopoietic Stem and Progenitor Cells. Current Protocols in Cytometry. 25:6.4:6.4.1–6.4.23.

Author Information

  1. 1

    The University Health Network, Toronto, Ontario, Canada

  2. 2

    London Health Sciences Center, London, Ontario, Canada

  3. 3

    Daniel den Hoed Cancer Center, Rotterdam, The Netherlands

Publication History

  1. Published Online: 1 AUG 2003
  2. Published Print: JUL 2003

Abstract

CD34 is the first documented cell surface antigen whose expression within the hematopoetic system is restricted to stem and progenitor cells of all lineages. Transplantation of CD34+ cells has created a need for accurate and rapid assessment of CD34+ cell concentrations in peripheral blood as well as for the optimal timing of apheresis sessions to insure the harvest of sufficient CD34+ cells. However, accurate enumeration of these cells represents a rare-event analysis, a demanding and somewhat complicated procedure. This comprehensive unit presents the clearest and most definitive methods yet available for CD34+ enumeration by flow cytometry, evaluates a number of commercially available procedures, and provides invaluable supporting information and detailed analysis which we believe is found in no other publication. All the possible problems are discussed and examples provided, allowing the clinical hematology lab to be certain that analysis of CD34+ cells is performed in an appropriate manner.

Keywords:

  • CD34;
  • hematopoietic stem cells;
  • enumeration;
  • multicolor analysis;
  • absolute counts;
  • viability staining