UNIT 6.5 Immunophenotypic Analysis of Peripheral Blood Lymphocytes
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Ruiz-Argüelles, A. and Pérez-Romano, B. 2001. Immunophenotypic Analysis of Peripheral Blood Lymphocytes. Current Protocols in Cytometry. 11:6.5:6.5.1–6.5.14.
- Published Online: 1 MAY 2001
- Published Print: JAN 2000
This unit on basic phenotyping describes two basic and two alternate protocols for the immunophenotypic identification and classification of human peripheral blood lymphocytes. The presented protocols comply with consensus recommendations from professional organizations that regulate the clinical use of such assays. The authors discuss whole blood assay systems as well as enriched systems from several perspectives, including absolute number determination.
Keywords: flow cytometry; peripheral blood; lymphocytes; immunophenotype; lymphocyte subsets