UNIT 6.5 Immunophenotypic Analysis of Peripheral Blood Lymphocytes

  1. Alejandro Ruiz-Argüelles,
  2. Beatriz Pérez-Romano

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0605s11

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Ruiz-Argüelles, A. and Pérez-Romano, B. 2001. Immunophenotypic Analysis of Peripheral Blood Lymphocytes. Current Protocols in Cytometry. 11:6.5:6.5.1–6.5.14.

Author Information

  1. Laboratorios Clínicos de Puebla, Puebla, Mexico

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: JAN 2000


This unit on basic phenotyping describes two basic and two alternate protocols for the immunophenotypic identification and classification of human peripheral blood lymphocytes. The presented protocols comply with consensus recommendations from professional organizations that regulate the clinical use of such assays. The authors discuss whole blood assay systems as well as enriched systems from several perspectives, including absolute number determination.

Keywords: flow cytometry; peripheral blood; lymphocytes; immunophenotype; lymphocyte subsets