UNIT 6.6 Immunophenotypic Analysis of Human Mast Cells by Flow Cytometry

  1. Luis Escribano,
  2. Raquel Navalón,
  3. Rosa Núñez,
  4. Beatriz Díaz Agustín,
  5. Pilar Bravo

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0606s12

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Escribano, L., Navalón, R., Núñez, R., Díaz Agustín, B. and Bravo, P. 2001. Immunophenotypic Analysis of Human Mast Cells by Flow Cytometry. Current Protocols in Cytometry. 12:6.6:6.6.1–6.6.18.

Author Information

  1. Hospital Ramón y Cajal, Mast Cell Unit, Madrid, Spain

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 2000


The immunophenotypic identification and enumeration of human bone marrow mast cells represents a clear demonstration of the utility of flow cytometry for rare-event analysis. The basic approach can be applied to a variety of specimens, including bone marrow, peripheral blood, ascitic fluid, and lymphoid tissue such as adenoids. Special emphasis is placed on markers with potential utility for distinguishing between normal, reactive, and pathological mast cells. From the clinical aspect, immunophenotypic analysis of mast cells may have great utility in supporting the diagnosis of tissue involvement in mastocytosis.