Unit

UNIT 6.11 Immunophenotypic Analysis of PNH Cells

  1. Stephen J. Richards,
  2. Peter Hillmen

Published Online: 1 MAY 2002

DOI: 10.1002/0471142956.cy0611s20

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Richards, S. J. and Hillmen, P. 2002. Immunophenotypic Analysis of PNH Cells. Current Protocols in Cytometry. 20:6.11:6.11.1–6.11.16.

Author Information

  1. Haematological Malignancy Diagnostic Service, Leeds, United Kingdom

Publication History

  1. Published Online: 1 MAY 2002
  2. Published Print: APR 2002

Abstract

Paroxysmal nocturnal hemoglobinuria (PNH) is a rare acquired hematopoietic stem-cell disorder in which a gene mutation results in cellular inability to synthesize the glycosylphosphatidylinositol (GPI) anchors needed to attach various proteins to the cell membrane. Traditional clinical laboratory PNH screening and diagnosis are based upon demonstration of increased erythrocyte susceptibility to lysis by activated complement. These methods are neither very specific nor very sensitive, and have largely been superseded by the rapid, sensitive, and specific flow cytometric analysis of GPI-linked antigen expression. The protocols in this unit cover immunophenotypic analysis of red blood cells, peripheral blood granulocytes, and peripheral blood monocytes, as well as a four-color modification for simultaneous analysis of peripheral blood granulocytes and monocytes.

Keywords:

  • PNH;
  • aplastic anemia;
  • GPI anchor;
  • CD55;
  • CD59;
  • hemolytic anemia;
  • immunophenotyping