Unit

UNIT 6.12 Quantitative Flow Cytometric Analysis of Membrane Antigen Expression

  1. Jean-Luc D'hautcourt

Published Online: 1 NOV 2002

DOI: 10.1002/0471142956.cy0612s22

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

D'hautcourt, J.-L. 2002. Quantitative Flow Cytometric Analysis of Membrane Antigen Expression. Current Protocols in Cytometry. 22:6.12:6.12.1–6.12.22.

Author Information

  1. Centre Hospitalier Régional Mons, Warquignies, Boussu, Belgium

Publication History

  1. Published Online: 1 NOV 2002
  2. Published Print: OCT 2002

Abstract

Immunological analysis for cell antigens has been performed by flow cytometry in a qualitative fashion for over thirty years. During that time it has become increasingly apparent that quantitative measurements such as number of antigens per cell provide unique and useful information. This unit on quantitative flow cytometry (QFCM) describes the most commonly used protocols, both direct and indirect, and the major methods of analysis for the number of antibody binding sites on a cell or particle. Practical applications include detection of antigen under- or overexpression in hematological malignancies, distinguishing between B cell lymphoproliferative disorders, and precise diagnosis of certain rare diseases.

Keywords:

  • flow cytometry;
  • quantitative flow cytometry (QFCM);
  • calibration;
  • fluorescence;
  • antibody binding capacity (ABC);
  • antibodies bound per cell (AB/C)