UNIT 6.12 Quantitative Flow Cytometric Analysis of Membrane Antigen Expression
Published Online: 1 NOV 2002
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
D'hautcourt, J.-L. 2002. Quantitative Flow Cytometric Analysis of Membrane Antigen Expression. Current Protocols in Cytometry. 22:6.12.1–6.12.22.
- Published Online: 1 NOV 2002
- Published Print: OCT 2002
Immunological analysis for cell antigens has been performed by flow cytometry in a qualitative fashion for over thirty years. During that time it has become increasingly apparent that quantitative measurements such as number of antigens per cell provide unique and useful information. This unit on quantitative flow cytometry (QFCM) describes the most commonly used protocols, both direct and indirect, and the major methods of analysis for the number of antibody binding sites on a cell or particle. Practical applications include detection of antigen under- or overexpression in hematological malignancies, distinguishing between B cell lymphoproliferative disorders, and precise diagnosis of certain rare diseases.
- flow cytometry;
- quantitative flow cytometry (QFCM);
- antibody binding capacity (ABC);
- antibodies bound per cell (AB/C)