UNIT 6.13 Immunophenotyping Using a Laser Scanning Cytometer
Published Online: 1 FEB 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Tárnok, A. and Gerstner, A. O. 2003. Immunophenotyping Using a Laser Scanning Cytometer. Current Protocols in Cytometry. 23:6.13:6.13.1–6.13.15.
- Published Online: 1 FEB 2003
- Published Print: JAN 2003
Three- and four-color immunophenotyping is routine in traditional flow cytometry, as is measurement of cell proliferation, but there are drawbacks. The techniques cannot analyze cell morphology or permit restaining of cells of interest. This unit describes a slide-based method of immunophenotyping using a laser scanning cytometer. In general, many assays originally developed for flow can be adapted to LSC. Although the speed of analysis is comparatively slow, LSC has the advantage that cells are not lost. Considerable additional information can be obtained by morphological examination and/or by further staining because specimens can be repeatedly analyzed and archived. The method has potential to become a powerful tool in clinical diagnosis.