UNIT 6.15 Whole Blood Analysis of Leukocyte-Platelet Aggregates
Published Online: 1 MAY 2003
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Barnard, M. R., Krueger, L. A., Frelinger, A., Furman, M. I. and Michelson, A. D. 2003. Whole Blood Analysis of Leukocyte-Platelet Aggregates. Current Protocols in Cytometry. 24:6.15:6.15.1–6.15.8.
- Published Online: 1 MAY 2003
- Published Print: APR 2003
In inflammatory and thrombotic syndromes, platelets aggregate with circulating leukocytes, especially monocytes and neutrophils. The platelet binding is initiated primarily through platelet surface expression of P-selectin (CD62P) following activation-dependent degranulation. The levels of P-selectin involved can be low enough to make direct measurement difficult, but detection of leukocyte-platelet aggregates is relatively simply by whole-blood flow cytometry. Light scatter and at least one leukocyte-specific antibody are used to gate the desired population, and the presence of associated platelets is detected by immunostaining for abundant platelet-specific markers.
- flow cytometry;
- coronary disease;
- activation-dependent receptors