Unit

UNIT 6.15 Whole Blood Analysis of Leukocyte-Platelet Aggregates

  1. Marc R. Barnard,
  2. Lori A. Krueger,
  3. A.L. Frelinger III,
  4. Mark I. Furman,
  5. Alan D. Michelson

Published Online: 1 MAY 2003

DOI: 10.1002/0471142956.cy0615s24

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Barnard, M. R., Krueger, L. A., Frelinger, A., Furman, M. I. and Michelson, A. D. 2003. Whole Blood Analysis of Leukocyte-Platelet Aggregates. Current Protocols in Cytometry. 24:6.15:6.15.1–6.15.8.

Author Information

  1. University of Massachusetts Medical School, Worcester, Massachusetts

Publication History

  1. Published Online: 1 MAY 2003
  2. Published Print: APR 2003

Abstract

In inflammatory and thrombotic syndromes, platelets aggregate with circulating leukocytes, especially monocytes and neutrophils. The platelet binding is initiated primarily through platelet surface expression of P-selectin (CD62P) following activation-dependent degranulation. The levels of P-selectin involved can be low enough to make direct measurement difficult, but detection of leukocyte-platelet aggregates is relatively simply by whole-blood flow cytometry. Light scatter and at least one leukocyte-specific antibody are used to gate the desired population, and the presence of associated platelets is detected by immunostaining for abundant platelet-specific markers.

Keywords:

  • flow cytometry;
  • leukocytes;
  • platelets;
  • P-selectin;
  • PSGL-1;
  • coronary disease;
  • activation-dependent receptors