UNIT 6.19 ZAP-70 Staining in Chronic Lymphocytic Leukemia

  1. Neus Villamor

Published Online: 1 MAY 2005

DOI: 10.1002/0471142956.cy0619s32

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Villamor, N. 2005. ZAP-70 Staining in Chronic Lymphocytic Leukemia. Current Protocols in Cytometry. 32:6.19:6.19.1–6.19.14.

Author Information

  1. Hospital Clínic de Barcelona, Barcelona, Spain

Publication History

  1. Published Online: 1 MAY 2005
  2. Published Print: APR 2005


Chronic lymphocytic leukemia (CLL) is the most common chronic leukemia in Western countries. The disease has an extremely variable clinical course, and several prognostic features have been identified to assess individual risk. The configuration of the immunoglobulin variable heavy-chain gene (IgVH) is a strong predictor of the outcome. CLL patients with unmutated IgVH status have an aggressive clinical course and a short survival. Unfortunately, analysis of IgVH gene configuration is not available in most clinical laboratories. A small number of genes are differentially expressed between unmutated IgVH and mutated IgVH clinical forms of CLL. One of these genes is ZAP-70, which is detected in leukemic cells from patients with the unmutated IgVH form of CLL. Flow cytometry presents advantages over other methods to detect ZAP-70, and its quantification by flow cytometry has proved its predictive value. This unit focuses on protocols to quantify ZAP-70 by flow cytometry in CLL.


  • ZAP-70;
  • chronic lymphocytic leukemia;
  • prognosis;
  • B lymphocytes;
  • mutational status of immunoglobulin variable heavy-chain gene