UNIT 6.25 Flow Cytometric Immunophenotyping of Cerebrospinal Fluid
Published Online: 1 JUL 2008
Copyright © 2008 by John Wiley & Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Kraan, J., Gratama, J. W., Haioun, C., Orfao, A., Plonquet, A., Porwit, A., Quijano, S., Stetler-Stevenson, M., Subira, D. and Wilson, W. 2008. Flow Cytometric Immunophenotyping of Cerebrospinal Fluid. Current Protocols in Cytometry. 45:6.25:6.25.1–6.25.16.
- Published Online: 1 JUL 2008
- Published Print: JUL 2008
Leptomeningeal disease is an important adverse complication occurring in patients with B and T cell lymphomas and acute leukemias of lymphoid and myeloid origin. Recent reports suggest that multiparameter flow cytometry immunophenotypic assessment of spinal fluid samples could improve the efficiency of detection of CNS involvement, due to its high specificity and greater sensitivity. However, spinal fluid samples are frequently paucicellular with a rapidly decreasing cell viability. Staining of spinal fluid therefore requires dedicated sample storage/transport, staining, and preparation protocols. The Basic Protocol in this unit outlines a consensus multiparameter (3- to 8-color) flow cytometry immunophenotypic protocol for the evaluation of CNS involvement of cerebrospinal fluid (CSF) samples by neoplastic cells. A Support Protocol describing the simultaneous assessment of surface and cytoplasmic antigens is also provided. Finally, in the Alternate Protocol, we describe a method to calculate absolute numbers of both normal and pathological cell subpopulations by adding counting beads to the assay. Curr. Protocol. Cytom. 45:6.25.1-6.25.16. © 2008 by John Wiley & Sons, Inc.
- cerebrospinal fluid;
- flow cytometry;