Unit

UNIT 6.26 Calibration of Flow Cytometry for Quantitative Quantum Dot Measurements

  1. Rowena Mittal1,3,
  2. Marcel P. Bruchez1,2,3

Published Online: 1 JUL 2009

DOI: 10.1002/0471142956.cy0626s49

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Mittal, R. and Bruchez, M. P. 2009. Calibration of Flow Cytometry for Quantitative Quantum Dot Measurements. Current Protocols in Cytometry. 49:6.26:6.26.1–6.26.7.

Author Information

  1. 1

    Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania

  2. 2

    Department of Chemistry, Carnegie Mellon University, Pittsburgh, Pennsylvania

  3. 3

    Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, Pennsylvania

Publication History

  1. Published Online: 1 JUL 2009
  2. Published Print: JUL 2009

Abstract

Observations of quantum dot (QD)–labeled cells in biomedical research are mainly qualitative in nature, which limits the ability of researchers to compare results experiment to experiment and laboratory to laboratory. Labeled cells are useful in a range of in vitro and in vivo assays where tracking behavior of administered cells is integral for answering research questions in areas such as tissue engineering and stem cell therapy. Before the full potential of QD-based toolsets can be realized, uptake of QDs by cells must be quantified and standardized. This unit describes a novel, simple method to assess the number of QDs per cell using flow cytometry and commercially available standards. This quick and easy method can be used to calibrate flow cytometry instruments and settings, and quantify QD uptake by cells for in vitro and in vivo experimentation for comparable results across QD conjugate types, cell types, research groups, lots of commercial QDs, and homemade QDs. Curr. Protoc. Cytom. 49:6.26.1-6.26.7. © 2009 by John Wiley & Sons, Inc.

Keywords:

  • quantitative;
  • quantum dot;
  • cell uptake;
  • flow cytometry;
  • number of quantum dots per cell;
  • QD uptake