Unit

UNIT 6.33 Three-Dimensional Second-Harmonic Generation Imaging of Fibrillar Collagen in Biological Tissues

  1. Jiansong Xie,
  2. John Ferbas,
  3. Gloria Juan

Published Online: 1 JUL 2012

DOI: 10.1002/0471142956.cy0633s61

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Xie, J., Ferbas, J. and Juan, G. 2012. Three-Dimensional Second-Harmonic Generation Imaging of Fibrillar Collagen in Biological Tissues. Current Protocols in Cytometry. 61:6.33:6.33.1–6.33.11.

Author Information

  1. Department of Medical Sciences, Amgen, Thousand Oaks, California

Publication History

  1. Published Online: 1 JUL 2012
  2. Published Print: JUL 2012

Abstract

Multiphoton-induced second-harmonic generation (SHG) has developed into a very powerful approach for in depth visualization of some biological structures with high specificity. In this unit, we describe the basic principles of three-dimensional SHG microscopy. In addition, we illustrate how SHG imaging can be utilized to assess collagen fibrils in biological tissues. Some technical considerations are also addressed. Curr. Protoc. Cytom. 61:6.33.1-6.33.11. © 2012 by John Wiley & Sons, Inc.

Keywords:

  • multiphoton microscopy;
  • second-harmonic generation;
  • laser scanning microscopy;
  • collagen;
  • extracellular matrix;
  • articular cartilage