Unit

UNIT 7.5 DNA Content Measurement for DNA Ploidy and Cell Cycle Analysis

  1. Zbigniew Darzynkiewicz,
  2. Gloria Juan

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0705s00

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Darzynkiewicz, Z. and Juan, G. 2001. DNA Content Measurement for DNA Ploidy and Cell Cycle Analysis. Current Protocols in Cytometry. 00:7.5:7.5.1–7.5.24.

Author Information

  1. New York Medical College, Valhalla, New York

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: APR 1997

Abstract

This unit presents the most common methods of DNA content analysis. The techniques differ primarily in the mode of cell permeabilization (detergents versus prefixation with alcohol) and composition of the stain solution. Protocols are given for analysis of fixed cells, tissue samples, live cells (for subsequent sorting and culture), archival paraffin-embedded tissue, and apoptotic cells.