UNIT 7.6 Analysis of Nuclear DNA Content and Ploidy in Higher Plants
Published Online: 1 MAY 2001
Copyright © 2003 by John Wiley and Sons, Inc.
Lab Protocol Title
Current Protocols in Cytometry
How to Cite
Galbraith, D. W., Lambert, G. M., Macas, J. and Dolezel, J. 2001. Analysis of Nuclear DNA Content and Ploidy in Higher Plants. Current Protocols in Cytometry. 2:7.6:7.6.1–7.6.22.
- Published Online: 1 MAY 2001
- Published Print: OCT 1997
This is the first of a series of units discussing the application of cytometry to plant material. Techniques commonly used for mammalian nuclei evaluation need considerable modification to be successful with plant material. David Galbraith and his colleagues bring together many years of knowledge in plant cytometry. Their unit provides detailed protocols on measuring DNA content, ploidy, and cell cycle status of plant tissue using both conventional laser based instruments as well as arc lamp cytometers. This unit provides an excellent starting point for those interested in doing cytometry with plants.