Unit

UNIT 7.6 Analysis of Nuclear DNA Content and Ploidy in Higher Plants

  1. David W. Galbraith1,
  2. Georgina M. Lambert1,
  3. Jiri Macas1,
  4. Jaroslav Dolezel2

Published Online: 1 MAY 2001

DOI: 10.1002/0471142956.cy0706s02

Current Protocols in Cytometry

Current Protocols in Cytometry

How to Cite

Galbraith, D. W., Lambert, G. M., Macas, J. and Dolezel, J. 2001. Analysis of Nuclear DNA Content and Ploidy in Higher Plants. Current Protocols in Cytometry. 2:7.6:7.6.1–7.6.22.

Author Information

  1. 1

    University of Arizona, Tucson, Arizona

  2. 2

    De Montfort University Norman Borlaug Centre for Plant Science, Olomouc, Czech Republic

Publication History

  1. Published Online: 1 MAY 2001
  2. Published Print: OCT 1997

Abstract

This is the first of a series of units discussing the application of cytometry to plant material. Techniques commonly used for mammalian nuclei evaluation need considerable modification to be successful with plant material. David Galbraith and his colleagues bring together many years of knowledge in plant cytometry. Their unit provides detailed protocols on measuring DNA content, ploidy, and cell cycle status of plant tissue using both conventional laser based instruments as well as arc lamp cytometers. This unit provides an excellent starting point for those interested in doing cytometry with plants.